|Year : 2022 | Volume
| Issue : 1 | Page : 7-9
Open-source smartphone adapter in digital photomicrography
Himel Mondal1, Shaikat Mondal2
1 Department of Physiology, Fakir Mohan Medical College and Hospital, Balasore, Odisha, India
2 Department of Physiology, Raiganj Government Medical College and Hospital, Raiganj, West Bengal, India
|Date of Submission||04-Jun-2020|
|Date of Decision||19-Aug-2020|
|Date of Acceptance||25-Aug-2020|
|Date of Web Publication||15-Feb-2022|
Dr. Shaikat Mondal
Department of Physiology, Raiganj Government Medical College and Hospital, Raiganj - 733 134, West Bengal
Source of Support: None, Conflict of Interest: None
Background and aim: Smartphones can be used to capture images from the microscope. There are commercial and homemade adapters that can be used to overcome the difficulty of focusing on a smartphone camera. We conducted this study to test if the usage of a homemade adapter reduces the time and effort of the operator in comparison to the free-hand technique in smartphone photomicrography. Materials and Methods: We made a simple smartphone adapter for digital photomicrography. Thirty-two operators first captured the image on the smartphone with the free-hand technique and then with the adapter thrice from a microscope. The time for focusing was compared statistically by paired t-test. A survey was conducted to know operators' opinions on adapter-assisted photomicrography. Result: All the participants were able to capture images from the microscopes. The average time for focusing with adapter was (11.89 ± 4.19 seconds) significantly (P = 0.0001) lower than the free-hand technique (25.56±11.81 seconds). However, the images yielded from both techniques were of equal quality. The majority of the participant found the method to be easy and reduce their effort in focusing and capturing an image. Conclusion: Low-cost homemade smartphone adapter helps in reducing the time required for capturing an image from the microscope. It decreases the effort of the operator in comparison to the free-hand technique. Hence, it may be a good choice for those who need to capture images from the microscopes frequently for either telemedicine or research purpose in resource-limited settings.
Keywords: Photomicrography; smartphone; microscopy; telemedicine
|How to cite this article:|
Mondal H, Mondal S. Open-source smartphone adapter in digital photomicrography. J Microsc Ultrastruct 2022;10:7-9
| Introduction|| |
Many of the resource-limited settings still do not have photomicrography enabled microscopes. For those settings, smartphone photomicrography would help in capturing images from the microscope for distant diagnosis.
Two techniques of smartphone photomicrography are prevalent- freehand and adapter-assisted. In the freehand technique, the operator positions the camera lens on the eyepiece to capture the image. In the adapter-assisted technique, the operator uses an adapter with the smartphone. An example is shown in [Figure 1]. There are various types of adapters that may help in smartphone photomicrography [Table 1].,,,,,
|Figure 1: Steps for focusing an image from a microscope with a smartphone aided with an adapter – (a) adapter with visible eyepiece casing and smartphone; (b) opposite side of the adapter and smartphone; (c) smartphone firmly held with the adapter; (d) eyepiece casing is brought to the lowest position; (e) slowly the combination of smartphone and the adapter is brought upward for focusing the image; (f) image is focused at a position and captured|
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|Table 1: Design of different types of smartphone adapter for digital photomicrography|
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Does usage of the adapter reduce the time and effort of focusing? The question is still unanswered in the literature.
| Aim|| |
This study aimed to find out if the usage of a smartphone adapter reduces the time and perceived effort of the operators.
| Methods|| |
This cross-sectional observational study was conducted after obtaining approval from Institutional Ethics Committee (Reference number: IEC/09/FAC/2019, dated 14 August 2019). All the research participants were recruited after obtaining written informed consent. All of them were above 18 years of age. We further declare that we conducted the study in accordance with the Declaration of Helsinki by the World Medical Association.
As the proprietary adapters are not procurable from all corners of the world, we used an open-source model for the test – simple smartphone adapter for digital photomicrography (sSADP). We conducted the study with 32 operators who captured the image of a focused object thrice from microscope eyepiece with the freehand technique first, then assisted with the adapter as shown in [Figure 1]. The time from starting the camera program to capture an image was stored for analysis. The participants were also asked to share their experience of focusing and capturing the image and their perception on the adapter in reducing their effort on a 5-point Likert-type scale.
Data were expressed in mean and standard deviation and tested statistically by paired t-test (for comparing the time of two techniques), one-way analysis of variance (for comparing episode-wise time in each technique), and Chi-square test (for the survey response) with α = 0.05 in GraphPad Prism software version 6.01 (GraphPad Software, CA, USA).
| Results|| |
There was noticeable less time required to capture the image by smartphone aided with the adapter when compared to the freehand technique [Table 2].
|Table 2: Comparison of time (seconds) required to capture image from a microscope by the freehand and the adapter-assisted technique (n=32)|
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Three images captured with a smartphone by the freehand technique are shown in [Figure 2]a, and the same field captured with the help of the adapter is shown in [Figure 2]b. Both had a similar quality.
|Figure 2: Photomicrographs of peripheral blood smears captured by smartphone camera with (a) freehand and (b) adapter-assisted technique. Footnote: images are cropped with 3:4 ratio from the center of photomicrographs with vignette and presented here without any modification|
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There was a reduction in time in both freehand and adapter-assisted technique with repeated measurement [Figure 3]. The majority of the participants found the focusing to be very easy with the sSADP and thinks that it reduced their effort in photomicrography [Table 3].
|Figure 3: Comparative time for freehand and adapter-assisted photomicrography in three episodes|
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| Discussion|| |
We found that the smartphone adapter helps in reducing the time for focusing through the microscope eyepiece in comparison to the freehand technique. Hence, researchers, academicians, and laboratory personnel from any resource-limited settings can use this type of homemade device for capturing images from the microscope with less time and effort. However, in both the techniques, focused images would be of equal quality.
The reason for the reduction in time may be attributed to the reduction of the number of axis for focusing. In the freehand technique, an operator needs to move her/his smartphone camera in X, Y, and Z-axis. If the operators use an adapter, movement in the X and Y axis is not required, and the only movement in the Z-axis is required.
| Conclusion|| |
Smartphone adapter-assisted photomicrography requires less time and effort than the freehand technique. However, both the methods yield similar image quality. Homemade and low-cost adapters may be built and used in resource-limited settings for academic and diagnostic purposes.
We thank Dr. Debasish Das, Department of Physiology and Dr. Sairavi Kiran Biri, Department of Biochemistry, Fakir Mohan Medical College and Hospital, Odisha, India, for their help in this study. We are grateful to the operators who participated in this study and took part in the survey.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Roy S, Pantanowitz L, Amin M, Seethala RR, Ishtiaque A, Yousem SA, et al
. Smartphone adapters for digital photomicrography. J Pathol Inform 2014;5:24.
] [Full text]
Mondal H, Mondal S, Das D. Development of a simple smartphone adapter for digital photomicrography. Indian Dermatol Online J 2017;8:485-6.
] [Full text]
Singaravel S, Aleem MA. Hands-free: A low-cost adapter for smartphone microscopic photography using a cardboard toilet-paper roll. Arch Pathol Lab Med 2016;140:741-3.
[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2], [Table 3]